Journal: Investigative Ophthalmology & Visual Science

Article Title: YBX1 Modulated Corneal Neovascularization Induced by Alkali Burn via m 5 C-Dependent Regulation of the STAT3/HIF-1α/VEGFA Axis

doi: 10.1167/iovs.67.2.42

Figure Lengend Snippet: YBX1 regulates angiogenesis and apoptosis in HUVECs through the HIF-1α pathway. ( A–H ) Western blot results and quantification of HIF-1α, VEGFA, Bcl-2, Bax, cleaved PARP, and cleaved Caspase-3 expression levels after overexpression of HIF-1α in the context of YBX1 knockdown. ( I ) TUNEL staining of control, H/R and different treatment groups evaluating by fluorescence microscope. Magnification = 40×; Scale bar = 50 µm. ( J ) Double ICC labeling for VEGFA and CD31 in HUVECs with YBX1 knockdown and HIF-1α overexpression. Magnification = 400×; Scale bar = 50 µm. ( K ) Scratch wound healing assay showing changes in cell migration ability among different treatment groups. Magnification = 100×; Scale bar = 500 µm. ( L ) Transwell migration assay assessing invasion capacity after YBX1 knockdown and HIF-1α overexpression. Magnification = 400×; Scale bar = 125 µm. * P < 0.05 versus the control group, # P < 0.05 versus the H/R group, ◆ P < 0.05 versus the H/R + siNC group, & P < 0.05 versus the H/R + siYBX1. Data are presented as mean ± SEM ( n = 6). Means were compared by 1-way ANOVA with Tukey's multiple comparison post hoc tests.

Article Snippet: Cell apoptosis was assessed via TUNEL staining (Vazyme, Cat #A112).

Techniques: Western Blot, Expressing, Over Expression, Knockdown, TUNEL Assay, Staining, Control, Fluorescence, Microscopy, Labeling, Wound Healing Assay, Migration, Transwell Migration Assay, Comparison

Journal: Cell

Article Title: Compromised SARS-CoV-2-specific placental antibody transfer

doi: 10.1016/j.cell.2020.12.027

Figure Lengend Snippet:

Article Snippet: Vector True View , Vector Laboratories , CAT # SP8400-15.

Techniques: Luciferase, Recombinant, Produced, Plasmid Preparation, Magnetic Beads, Software, Luminex